A neosporose tem grande importância em bovinos, e sua patogênese neste hospedeiro, em termos gerais, está esclarecida, porém, em equídeos é pobremente conhecida. Na Colômbia ainda não foram reportados anticorpos Anti-Neospora spp. em equídeos, sendo assim objetivou-se no presente estudo avaliar a ocorrência do parasito em jumentos (Equus asinus) de fazendas que apresentavam risco da doença nestes animais. Foram utilizados 56 animais no estado de Sucre (Colômbia), escolhidos aleatoriamente dentro das fazendas selecionadas. Utilizou-se um peptídeo recombinante originado de Neospora caninum (NcGRA1) para o diagnóstico por Dot-ELISA, e o soro foi diluído em 1:200. Este estudo reporta, pela primeira vez no estado de Sucre e na Colômbia, a presença de anticorpos anti-Neospora spp. na espécie Equus asinus, com uma ocorrência de 19,7% (11/56) dos animais amostrados.

The neosporosis has great importance in cattle, and its pathogenesis in this host has been generally clarified, however, in horses, neosporosis is poorly known, and in Colombia anti-Neospora spp antibodies have not been reported. Therefore, the main objective in the present study was to evaluate the occurrence of this parasite in donkeys (Equus asinus) from farms that presented a risk of disease in these animals, as well as no health plan for them. Were used 56 animals randomly chosen inside selected farms in the state of Sucre (Colombia). A recombinant peptide originated from Neospora caninum (NcGRA1) was used for the diagnosis with Dot-ELISA and serum was diluted 1:200. This study is the first to report the presence of anti-Neospora spp. in donkeys (Equus asinus) in the state of Sucre, and in Colombia. The occurrence was in 19.7% of the animals sampled (11/56).

Zinc-blende GaAs layers were prepared on (100) Si and glass substrates by r.f. magnetron sputtering. The morphology of GaAs layers is analyzed by means of atomic force microscopy (AFM) and scanning electron microscopy (FE-SEM), to determine the sample topography and growth type. The compositional analysis was performed by means of energy dispersive X-ray spectroscopy (EDS), in order to obtain information of the atomic percentages of the elements and their spatial distribution in the samples. The optical properties of the layers are discussed from the results of UV-Vis absorption, and Photoacoustic spectroscopy (PAS). Finally, the Raman shift of the GaAs phonon modes are studied as function of the penetration depth of laser wavelength used to excite the sample on Raman microscopy.